Atletico Madrid striker Diego Costa will serve his eight-match ban after the appeal over his suspension was thrown out by the Royal Spanish Football Federation (RFEF).
Spanish giants Atletico had earlier appealed against the sentence given to the former Chelsea player only for the appeal to be thrown out by the football federation.
Diego Costa was handed an eight-match ban after he used foul words against centre referee Jesus Gil Manzano during the top-of-the-table clash with Barcelona in the Spanish La Liga last weekend.
The Brazilian-born Spanish international striker was sent off in the 28th minute for his rant towards the referee and that ultimately cost his team the three points at stake.
Atletico Madrid were looking to reduce the gap to Barcelona at the top of the Spanish La Liga table to five points with a win at the Nou Camp only for them to suffer the Diego Costa setback.
Atletico Madrid played the majority of the away league game at Barcelona with ten men and went on to lose 2-0 courtesy two goals within a minute from the attacking duo of Lionel Messi and Luis Suarez.
Both goals came in the final ten minutes of the game as Barcelona extended their lead at the La Liga summit to eleven points.